ELIZA cgi-bash version rev. 1.90
- Medical English LInking keywords finder for the PubMed Zipped Archive (ELIZA) -

return kwic search for cells out of >500 occurrences
856246 occurrences (No.4 in the rank) during 5 years in the PubMed. [no cache] 500 found
5) Activation of the PI3K-AKT pathway and, to a lesser extent, the MEK-ERK pathway appears to be required for this process, as blocking with specific inhibitors interferes with the differentiation of K562 cells.
--- ABSTRACT ---
PMID:23166016 DOI:10.1002/term.1628
2015 Journal of tissue engineering and regenerative medicine
* 2-(trimethylammonium)ethyl (R)-3-methoxy-3-oxo-2-stearamidopropyl phosphate promotes megakaryocytic differentiation of myeloid leukaemia cells and primary human CD34⁺ haematopoietic stem cells.
- In this study we showed that 2-(trimethylammonium)ethyl (R)-3-methoxy-3-oxo-2-stearamidopropyl phosphate [(R)-TEMOSPho], a derivative of an organic chemical identified from a natural product library, promotes highly efficient differentiation of megakaryocytes. Specifically, (R)-TEMOSPho induces cell cycle arrest, cell size increase and polyploidization from K562 and HEL cells, which are used extensively to model megakaryocytic differentiation. In addition, megakaryocyte-specific cell surface markers showed a dramatic increase in expression in response to (R)-TEMOSPho treatment. Importantly, we demonstrated that such megakaryocytic differentiation can also be induced from primary human CD34(+) haematopoietic stem cells. Activation of the PI3K-AKT pathway and, to a lesser extent, the MEK-ERK pathway appears to be required for this process, as blocking with specific inhibitors interferes with the differentiation of K562 cells. A subset of (R)-TEMOSPho-treated K562 cells undergoes spontaneous apoptosis and produces platelets that are apparently functional, as they bind to fibrinogen, express P-selectin and aggregate in response to SFLLRN and AYPGFK, the activating peptides for the PAR1 and PAR4 receptors, respectively. Taken together, these results indicate that (R)-TEMOSPho will be useful for dissecting the molecular mechanisms of megakaryocytic differentiation, and that this class of compounds represents potential therapeutic reagents for thrombocytopenia.
--- ABSTRACT END ---
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[frequency of next (right) word to cells]
(1)80 *null* (15)5 as (29)3 exposed (43)2 expanded
(2)47 and (16)5 expressed (30)3 have (44)2 expressing
(3)39 were (17)5 of (31)3 isolated (45)2 had
(4)26 in (18)5 which (32)3 showed (46)2 labeled
(5)12 (MSCs) (19)4 (ASCs) (33)3 we (47)2 may
(6)11 was (20)4 has (34)2 (ASCs), (48)2 play
(7)10 to (21)4 is (35)2 (BMSCs) (49)2 present
(8)9 with (22)4 on (36)2 (ECFCs) (50)2 remains
(9)7 are (23)4 seeded (37)2 (HSCs) (51)2 such
(10)6 can (24)4 within (38)2 after (52)2 the
(11)6 for (25)3 (ASC) (39)2 by (53)2 via
(12)6 from (26)3 (ECs) (40)2 caused
(13)6 or (27)3 (HUVECs) (41)2 cultured
(14)6 that (28)3 (hMSCs) (42)2 derived

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--- WordNet output for cells --- Overview of noun cell The noun cell has 7 senses (first 3 from tagged texts) 1. (71) cell -- (any small compartment; "the cells of a honeycomb") 2. (44) cell -- ((biology) the basic structural and functional unit of all organisms; they may exist as independent units of life (as in monads) or may form colonies or tissues as in higher plants and animals) 3. (1) cell, electric cell -- (a device that delivers an electric current as the result of a chemical reaction) 4. cell, cadre -- (a small unit serving as part of or as the nucleus of a larger political movement) 5. cellular telephone, cellular phone, cellphone, cell, mobile phone -- (a hand-held mobile radiotelephone for use in an area divided into small sections, each with its own short-range transmitter/receiver) 6. cell, cubicle -- (small room in which a monk or nun lives) 7. cell, jail cell, prison cell -- (a room where a prisoner is kept) --- WordNet end ---