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270181 occurrences (No.97 in the rank) during 5 years in the PubMed. [cache]
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270181 occurrences (No.97 in the rank) during 5 years in the PubMed. [cache]
| 317) Ex vivo whole blood stimulation with MPLA revealed a lower induction of the TNFα secreted protein in 20 septic patients (SP) compared to 10 healthy volunteers (HV), in agreement with monocyte anergy. |
| PMID:33741504 DOI:10.1016/j.clim.2021.108714 |
| 2021 Clinical immunology (Orlando, Fla.) |
| * Differential response induced by LPS and MPLA in immunocompetent and septic individuals. |
| - Lipopolysaccharide (LPS) and monophosphoryl lipid A (MPLA) induce, overall, similar transcriptional profiles in healthy individuals, although LPS has been shown to more potently induce pro-inflammatory cytokines. We explore herein whether MPLA could be considered as a synthetic replacement of LPS in immune functional assays to study anergy of immune cells in septic patients. Ex vivo whole blood stimulation with MPLA revealed a lower induction of the TNFα secreted protein in 20 septic patients (SP) compared to 10 healthy volunteers (HV), in agreement with monocyte anergy. Principal component analysis of the 93-gene molecular response to MPLA and LPS stimulation found that the main variability was driven by stimulation in HV and by pathophysiology in SP. MPLA was a stronger inducer of the HLA family genes than LPS in both populations, arguing for divergent signalling pathways downstream of TLR-4. In addition, MPLA appeared to present a more informative stratification potential within the septic population. |
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(15)5 placebo |
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(41)2 patients |
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(16)5 that |
(29)2 PLWH |
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(17)4 standard |
(30)2 TM |
(43)2 poor |
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(18)4 women |
(31)2 TomTec |
(44)2 swabs |
| (6)13 controls |
(19)3 group |
(32)2 WT |
(45)2 therapy |
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(20)3 males |
(33)2 allogeneic |
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| (8)7 baseline |
(21)3 no |
(34)2 any |
(47)2 uninfected |
| (9)7 their |
(22)3 non-obese |
(35)2 children |
(48)2 untreated |
| (10)6 control, |
(23)3 wild-type |
(36)2 expert |
(49)2 usual |
| (11)6 individuals |
(24)2 B-385 |
(37)2 females |
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| (12)6 normal |
(25)2 CON |
(38)2 field |
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| (13)5 controls, |
(26)2 CS- |
(39)2 irradiated |
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--- WordNet output for compared ---