2015 Journal of tissue engineering and regenerative medicine
* Hydrogels as feeder-free scaffolds for long-term self-renewal of mouse induced pluripotent stem cells.
- Expanding undifferentiated induced pluripotent stem (iPS) cells in vitro is a basic requirement for application of iPS cells in both fundamental research and clinical regeneration. In this study, we intended to establish a simple, low cost and efficient method for the long-term self-renewal of mouse induced pluripotent stem (miPS) cells without using feeder-cells and adhesive proteins. Three scaffolds were selected for the long-term subculture of miPS cells over two months starting from passages 14 to 29: 1) a gelatin coated polystyrene (Gelatin-PS) that is a widely used scaffold for self-renewal of mouse embryonic stem (mES) cells; 2) a neutral hydrogel poly(N,N-dimethylacrylamide) (PDMAAm); and 3) a negatively charged hydrogel poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS). Each passaged miPS cells on these scaffolds were cryopreserved successfully and the revived cells showed high viability and proliferation. The passaged miPS cells maintained a high undifferentiated state on all three scaffolds and a high level of pluripotency by expressing differentiation markers in vitro and forming teratomas in SCID mice with derivatives of all three germ layers. Compared to Gelatin-PS, the two hydrogels exhibited much better self-renewal performance in terms of high proliferation rate and level of expression of undifferentiated gene markers as well as efficiency in pluripotent teratoma formation. Furthermore, the PNaAMPS hydrogel demonstrated a slightly higher efficiency and simpler operation of cell expansion than the PDMAAm hydrogel. To conclude, PNaAMPS hydrogel is an excellent feeder-free scaffold because of its simplicity, low cost and high efficiency in expanding a large number of miPS cells in vitro.
Overview of verb demonstrate
The verb demonstrate has 4 senses (first 4 from tagged texts)
1. (24) show, demo, exhibit, present, demonstrate -- (give an exhibition of to an interested
audience; "She shows her dogs frequently"; "We will demo the new software in Washington")
2. (22) prove, demonstrate, establish, show, shew -- (establish the validity of something, as by an
example, explanation or experiment; "The experiment demonstrated the instability of the compound";
"The mathematician showed the validity of the conjecture")
3. (2) attest, certify, manifest, demonstrate, evidence -- (provide evidence for; stand as proof of;
show by one's behavior, attitude, or external attributes; "His high fever attested to his illness";
"The buildings in Rome manifest a high level of architectural sophistication"; "This decision
demonstrates his sense of fairness")
4. (1) demonstrate, march -- (march in protest; take part in a demonstration; "Thousands
demonstrated against globalization during the meeting of the most powerful economic nations in
Seattle")
Overview of adj demonstrated
The adj demonstrated has 1 sense (first 1 from tagged texts)
1. (1) demonstrated -- (having been demonstrated or verified beyond doubt)
--- WordNet end ---