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383385 occurrences (No.47 in the rank) during 5 years in the PubMed. [no cache] 500 found

7) The PCR products amplified from the extracted mouse eye DNA confirmed the development of Acanthamoeba-induced keratitis during the infection periods.

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PMID:33603075 DOI:10.1038/s41598-021-83738-4

2021 Scientific reports

* Establishment of an Acanthamoeba keratitis mouse model confirmed by amoebic DNA amplification.

- Acanthamoeba castellanii, the causative agent of Acanthamoeba keratitis (AK), occurs mainly in contact lens users with poor eye hygiene. The findings of many in vitro studies of AK, as well as the testing of therapeutic drugs, need validation in in vivo experiments. BALB/c mice were used in this study to establish in vivo AK model. A. castellanii cell suspensions (equal mixtures of trophozoites and cysts) were loaded onto 2-mm contact lens pieces and inserted into mouse eyes that were scratched using an ophthalmic surgical blade under anesthesia and the eyelids of the mice were sutured. The AK signs were grossly observed and PCR was performed using P-FLA primers to amplify the Acanthamoeba 18S-rRNA gene from mouse ocular tissue. The experimental AK mouse model was characterized by typical hazy blurring and melting of the mouse cornea established on day 1 post-inoculation. AK was induced with at least 0.3 × 10⁵ A. castellanii cells (optimal number, 5 × 10⁴), and the infection persisted for two months. The PCR products amplified from the extracted mouse eye DNA confirmed the development of Acanthamoeba-induced keratitis during the infection periods. In conclusion, the present AK mouse model may serve as an important in vivo model for the development of various therapeutic drugs against AK.

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(1)207 of	(6)5 is	(11)3 process	(16)2 process,
(2)74 and	(7)5 to	(12)2 aid	(17)2 programmes
(3)68 null	(8)4 has	(13)2 but	(18)2 programs
(4)13 in	(9)4 the	(14)2 for	(19)2 research
(5)5 Goals	(10)3 are	(15)2 implementation	(20)2 studies

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--- WordNet output for development --- =>発達, 開発, 産物, 進展 Overview of noun development The noun development has 9 senses (first 7 from tagged texts) 1. (52) development -- (act of improving by expanding or enlarging or refining; "he congratulated them on their development of a plan to meet the emergency"; "they funded research and development") 2. (46) development, evolution -- (a process in which something passes by degrees to a different stage (especially a more advanced or mature stage); "the development of his ideas took many years"; "the evolution of Greek civilization"; "the slow development of her skill as a writer") 3. (11) growth, growing, maturation, development, ontogeny, ontogenesis -- ((biology) the process of an individual organism growing organically; a purely biological unfolding of events involved in an organism changing gradually from a simple to a more complex level; "he proposed an indicator of osseous development in children") 4. (9) development -- (a recent event that has some relevance for the present situation; "recent developments in Iraq"; "what a revolting development!") 5. (7) exploitation, development -- (the act of making some area of land or water more profitable or productive or useful; "the development of Alaskan resources"; "the exploitation of copper deposits") 6. (6) development -- (a district that has been developed to serve some purpose; "such land is practical for small park developments") 7. (4) development -- (a state in which things are improving; the result of developing (as in the early part of a game of chess); "after he saw the latest development he changed his mind and became a supporter"; "in chess your should take care of your development before moving your queen") 8. development, developing -- (processing a photosensitive material in order to make an image visible; "the development and printing of his pictures took only two hours") 9. development -- ((music) the section of a composition or movement (especially in sonata form) where the major musical themes are developed and elaborated) --- WordNet end ---