ELIZA cgi-bash version rev. 1.91
- Medical English LInking keywords finder for the PubMed Zipped Archive (ELIZA) -

return kwic search for expression of out of >500 occurrences
286252 occurrences (No.88 in the rank) during 5 years in the PubMed. [cache]
218) HT29 colon cancer cells transfected with the CaSR were treated with both enantiomers of NPS 568 and NPS 2143 alone or in combination, and the expression of CaSR and the pro-inflammatory cytokine interleukin 8 (IL-8) was measured by RT-qPCR and ELISA.
--- ABSTRACT ---
PMID:34576291 DOI:10.3390/ijms221810124
2021 International journal of molecular sciences
* Stereo-Specific Modulation of the Extracellular Calcium-Sensing Receptor in Colon Cancer Cells.
- Pharmacological allosteric agonists (calcimimetics) of the extracellular calcium-sensing receptor (CaSR) have substantial gastro-intestinal side effects and induce the expression of inflammatory markers in colon cancer cells. Here, we compared the effects of both CaSR-specific (R enantiomers) and -unspecific (S enantiomers) enantiomers of a calcimimetic (NPS 568) and a calcilytic (allosteric CaSR antagonists; NPS 2143) to prove that these effects are indeed mediated via the CaSR, rather than via off-target effects, e.g., on β-adrenoceptors or calcium channels, of these drugs. The unspecific S enantiomer of NPS 2143 and NPS S-2143 was prepared using synthetic chemistry and characterized using crystallography. NPS S-2143 was then tested in HEK-293 cells stably transfected with the human CaSR (HEK-CaSR), where it did not inhibit CaSR-mediated intracellular Ca2+ signals, as expected. HT29 colon cancer cells transfected with the CaSR were treated with both enantiomers of NPS 568 and NPS 2143 alone or in combination, and the expression of CaSR and the pro-inflammatory cytokine interleukin 8 (IL-8) was measured by RT-qPCR and ELISA. Only the CaSR-selective enantiomers of the calcimimetic NPS 568 and NPS 2143 were able to modulate CaSR and IL-8 expression. We proved that pro-inflammatory effects in colon cancer cells are indeed mediated through CaSR activation. The non-CaSR selective enantiomer NPS S-2143 will be a valuable tool for investigations in CaSR-mediated processes.
--- ABSTRACT END ---
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[frequency of next (right) word to expression of]
(1)32 the (13)4 mutant (24)2 KIF20B (36)2 heat
(2)18 genes (14)4 specific (25)2 Ki-67 (37)2 miR-296
(4)8 a (15)3 many (26)2 MMP-1 (38)2 miR-627
(5)7 these (16)3 mitochondrial (27)2 NCS1 (39)2 miRNAs
(6)6 nuclear (17)3 multiple (29)2 SLCO2B1 (40)2 oncoproteins,
(7)5 Staphylococcus (18)3 several (30)2 VEGF (41)2 platelet-derived
(8)5 pro-inflammatory (19)3 vascular (31)2 asSuGR, (42)2 recombinant
(9)4 CYP3A4 (20)2 ACE-2 (32)2 cytokines/chemokines (43)2 tight
(10)4 both (21)2 ASIC1a, (33)2 farnesoid
(11)4 inflammatory (22)2 ATG5 (34)2 fibroblast
(12)4 its (23)2 CCN1 (35)2 fibrogenic

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--- WordNet output for expression --- =>表現すること, 式, 表現, 表示, 言い回し, 表現法, 表情 Overview of noun expression The noun expression has 9 senses (first 6 from tagged texts) 1. (23) expression, look, aspect, facial expression, face -- (the feelings expressed on a person's face; "a sad expression"; "a look of triumph"; "an angry face") 2. (18) expression, manifestation, reflection, reflexion -- (expression without words; "tears are an expression of grief"; "the pulse is a reflection of the heart's condition") 3. (15) expression, verbal expression, verbalism -- (the communication (in speech or writing) of your beliefs or opinions; "expressions of good will"; "he helped me find verbal expression for my ideas"; "the idea was immediate but the verbalism took hours") 4. (5) saying, expression, locution -- (a word or phrase that particular people use in particular situations; "pardon the expression") 5. (4) formulation, expression -- (the style of expressing yourself; "he suggested a better formulation"; "his manner of expression showed how much he cared") 6. (4) formula, expression -- (a group of symbols that make a mathematical statement) 7. expression -- ((genetics) the process of expressing a gene) 8. construction, grammatical construction, expression -- (a group of words that form a constituent of a sentence and are considered as a single unit; "I concluded from his awkward constructions that he was a foreigner") 9. expression -- (the act of forcing something out by squeezing or pressing; "the expression of milk from her breast") --- WordNet end ---