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10) Consequently, sequencing results obtained from the nad1 gene as a mitochondrial marker for the first time in this study would provide a valuable tool to analyse further molecular details of D.
* Characterization of Dicrocoelium dendriticum haplotypes from sheep and cattle in Iran based on the internal transcribed spacer 2 (ITS-2) and NADH dehydrogenase gene (nad1).
- The present study assessed whether the genetic variation among different hosts (sheep and cattle) and geographical isolates (n= 28) of Dicrocoelium dendriticum from Iran is present based on mitochondrial (nad1) and ribosomal (ITS-2) DNA markers. Molecular analysis revealed the presence of at least ten and two distinct haplotypes in the NADH dehydrogenase gene (nad1) and internal transcribed spacer 2 (ITS-2), respectively. The nad1 and ITS-2 sequence data were deposited in GenBank under accession numbers, JX050110-134 and JQ966972-3. According to the results of our study, ND-D and ITS-A are established as being the predominant haplotypes of D. dendriticum in Iran. The Iranian isolates showed a higher intraspecific genetic diversity of 0-0.97% for nad1, compared to 0-0.42% for ITS-2. The alignment and comparison of nad1 and ITS-2 sequences revealed eight and one polymorphic sites, respectively. In the nad1 sequences, six were silent and two nucleotide substitutions were responsible for amino acid alterations. A phylogenetic analysis of the sequence data revealed that host associations and geographic location are not likely useful markers for D. dendriticum haplotype classification. Consequently, sequencing results obtained from the nad1 gene as a mitochondrial marker for the first time in this study would provide a valuable tool to analyse further molecular details of D. dendriticum worldwide.
=>初めて, 最初の, 第一の, はじめに, 最初, 第1
Overview of noun first
The noun first has 6 senses (first 4 from tagged texts)
1. (9) first, number one -- (the first or highest in an ordering or series; "He wanted to be the
first")
2. (5) first, number one, number 1 -- (the first element in a countable series; "the first of the
month")
3. (1) beginning, commencement, first, outset, get-go, start, kickoff, starting time, showtime,
offset -- (the time at which something is supposed to begin; "they got an early start"; "she knew
from the get-go that he was the man for her")
4. (1) first base, first -- (the fielding position of the player on a baseball team who is stationed
at first of the bases in the infield (counting counterclockwise from home plate))
5. first, first-class honours degree -- (an honours degree of the highest class)
6. first gear, first, low gear, low -- (the lowest forward gear ratio in the gear box of a motor
vehicle; used to start a car moving)
Overview of adj first
The adj first has 6 senses (first 5 from tagged texts)
1. (232) first -- (preceding all others in time or space or degree; "the first house on the right";
"the first day of spring"; "his first political race"; "her first baby"; "the first time"; "the
first meetings of the new party"; "the first phase of his training")
2. (61) first, 1st -- (indicating the beginning unit in a series)
3. (4) inaugural, initiative, initiatory, first, maiden -- (serving to set in motion; "the
magazine's inaugural issue"; "the initiative phase in the negotiations"; "an initiatory step toward
a treaty"; "his first (or maiden) speech in Congress"; "the liner's maiden voyage")
4. (4) beginning, first -- (serving to begin; "the beginning canto of the poem"; "the first verse")
5. (3) first, foremost, world-class -- (ranking above all others; "was first in her class"; "the
foremost figure among marine artists"; "the top graduate")
6. first -- (highest in pitch or chief among parts or voices or instruments or orchestra sections;
"first soprano"; "the first violin section"; "played first horn")
Overview of adv first
The adv first has 4 senses (first 4 from tagged texts)
1. (58) first, firstly, foremost, first of all, first off -- (before anything else; "first we must
consider the garter snake")
2. (40) first, for the first time -- (the initial time; "when Felix first saw a garter snake")
3. (9) first -- (before another in time, space, or importance; "I was here first"; "let's do this
job first")
4. (4) foremost, first -- (prominently forward; "he put his best foot foremost")
--- WordNet end ---