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378430 occurrences (No.48 in the rank) during 5 years in the PubMed. [no cache] 500 found

215) First, the immunophenotyping properties and potential of MenSCs to differentiate into osteoblasts, adipocytes and chondrocytes were evaluated.

--- ABSTRACT ---

PMID:23505217 DOI:10.1002/term.1715

2015 Journal of tissue engineering and regenerative medicine

* Efficient generation of functional hepatocyte-like cells from menstrual blood-derived stem cells.

- In recent years, the advantages of menstrual blood-derived stem cells (MenSCs), such as minimal ethical considerations, easy access and high proliferative ability, have inspired scientists to investigate the potential of MenSCs in cell therapy of different diseases. In order to characterize the potency of these cells for future cell therapy of liver diseases, we examined the potential of MenSCs to differentiate into hepatocytes, using different protocols. First, the immunophenotyping properties and potential of MenSCs to differentiate into osteoblasts, adipocytes and chondrocytes were evaluated. Thereafter, the differentiation protocols developed by two concentrations of hepatocyte growth factor (HGF) and oncostatin M (OSM), in combination with other components in serum-supplemented or serum-free culture media, were also investigated. The sequential differentiation was monitored by real-time PCR, immunostaining and functional assays. Our primary data revealed that the isolated MenSCs exhibited mesenchymal stem cell markers in parallel to OCT-4 as an embryonic marker. Regardless of differentiation procedures, the developed cells expressed mature hepatocyte markers, such as albumin, tyrosine aminotransferase and cytokeratin-18 at the mRNA and protein levels. They also showed functional properties of hepatocytes, including albumin secretion, glycogen storage and cytochrome P450 7A1 expression. However, the degree of differentiation was dependent on the concentrations of HGF and OSM. Indeed, omission of serum during the differentiation process caused typical improvement in hepatocyte-specific functions. This study is a novel report demonstrating the differentiation potential of MenSCs into hepatocyte-like cells. We recommend a complementary serum-free differentiation protocol for enrichment of in vitro production of functional MenSC-derived hepatocyte-like cells that could lead to a major step toward applied stem cell therapy of chronic liver diseases.

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(1)32 time	(19)5 meaningful	(37)2 5	(55)2 marriage
(2)21 report	(20)4 line	(38)2 6	(56)2 meta-analysis
(3)11 and	(21)3 14	(39)2 72	(57)2 mitochondrial
(4)11 to	(22)3 3	(40)2 application	(58)2 months
(5)10 time,	(23)3 complete	(41)2 approach	(59)2 myocardial
(6)9 year	(24)3 described	(42)2 article	(60)2 postnatal
(7)8 2	(25)3 in	(43)2 attempt	(61)2 presented
(8)8 molar	(26)3 it	(44)2 choice	(62)2 relapse
(9)8 reported	(27)3 order	(45)2 clinical	(63)2 smear
(10)8 step	(28)3 postoperative	(46)2 component	(64)2 stage
(11)8 we	(29)3 record	(47)2 comprehensive	(65)2 step,
(12)7 case	(30)3 sequenced	(48)2 concern	(66)2 test
(13)6 determined	(31)3 the	(49)2 course	(67)2 that
(14)6 group	(32)3 treatment	(50)2 demonstration	(68)2 therapy
(15)6 study	(33)3 two	(51)2 few	(69)2 trimester
(16)5 dose	(34)3 was	(52)2 intentions	(70)2 trimester,
(17)5 evidence	(35)2 null	(53)2 introduction	(71)2 using
(18)5 generation	(36)2 30	(54)2 left

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--- WordNet output for first --- =>初めて, 最初の, 第一の, はじめに, 最初, 第1 Overview of noun first The noun first has 6 senses (first 4 from tagged texts) 1. (9) first, number one -- (the first or highest in an ordering or series; "He wanted to be the first") 2. (5) first, number one, number 1 -- (the first element in a countable series; "the first of the month") 3. (1) beginning, commencement, first, outset, get-go, start, kickoff, starting time, showtime, offset -- (the time at which something is supposed to begin; "they got an early start"; "she knew from the get-go that he was the man for her") 4. (1) first base, first -- (the fielding position of the player on a baseball team who is stationed at first of the bases in the infield (counting counterclockwise from home plate)) 5. first, first-class honours degree -- (an honours degree of the highest class) 6. first gear, first, low gear, low -- (the lowest forward gear ratio in the gear box of a motor vehicle; used to start a car moving) Overview of adj first The adj first has 6 senses (first 5 from tagged texts) 1. (232) first -- (preceding all others in time or space or degree; "the first house on the right"; "the first day of spring"; "his first political race"; "her first baby"; "the first time"; "the first meetings of the new party"; "the first phase of his training") 2. (61) first, 1st -- (indicating the beginning unit in a series) 3. (4) inaugural, initiative, initiatory, first, maiden -- (serving to set in motion; "the magazine's inaugural issue"; "the initiative phase in the negotiations"; "an initiatory step toward a treaty"; "his first (or maiden) speech in Congress"; "the liner's maiden voyage") 4. (4) beginning, first -- (serving to begin; "the beginning canto of the poem"; "the first verse") 5. (3) first, foremost, world-class -- (ranking above all others; "was first in her class"; "the foremost figure among marine artists"; "the top graduate") 6. first -- (highest in pitch or chief among parts or voices or instruments or orchestra sections; "first soprano"; "the first violin section"; "played first horn") Overview of adv first The adv first has 4 senses (first 4 from tagged texts) 1. (58) first, firstly, foremost, first of all, first off -- (before anything else; "first we must consider the garter snake") 2. (40) first, for the first time -- (the initial time; "when Felix first saw a garter snake") 3. (9) first -- (before another in time, space, or importance; "I was here first"; "let's do this job first") 4. (4) foremost, first -- (prominently forward; "he put his best foot foremost") --- WordNet end ---