391) A metagenomic xylanase gene (Mxyl) was successfully cloned into shuttle vector pWH1520 and expressed in Bacillus subtilis extracellularly. |
PMID:24124029 DOI:10.1002/btpr.1826 |
2015 Biotechnology progress |
* Production of cellulase-free xylanase by the recombinant Bacillus subtilis and its applicability in paper pulp bleaching. |
- A metagenomic xylanase gene (Mxyl) was successfully cloned into shuttle vector pWH1520 and expressed in Bacillus subtilis extracellularly. On induction with xylose, recombinant xylanase secretion commenced after 6 h. Identifying critical variables for recombinant xylanase production by one-variable-at-time approach followed by optimization of the selected variables (xylose, inoculum density, incubation density) by response surface methodology (RSM) led to three-fold enhancement in extracellular xylanase production (119 U mL(-1) ). When the pulp was treated with recombinant xylanase at 80°C and pH 9.0, kappa number of the pulp was reduced with concomitant increase in brightness and 24% reduction in chlorine consumption. This is the first report on the expression of metagenomic xylanase gene in Bacillus subtilis extracellularly and its utility in developing an environment-friendly pulp bleaching process. |
(1)75 expression | (15)5 of | (29)3 block | (43)2 mutation |
(2)34 order | (16)5 transfer | (30)3 can | (44)2 on |
(3)32 arrangement | (17)5 were | (31)3 contents | (45)2 overlap |
(4)31 and | (18)4 activation | (32)3 polymorphisms | (46)2 peptide |
(5)21 *null* | (19)4 delivery | (33)3 sequencing | (47)2 profiling |
(6)15 was | (20)4 encoding | (34)3 transcription | (48)2 regions |
(7)12 expression, | (21)4 expressions | (35)2 analysis | (49)2 sequence |
(8)9 arrangement, | (22)4 fragments | (36)2 carriers | (50)2 sequences, |
(9)7 composition | (23)4 is | (37)2 cluster | (51)2 spookier |
(10)7 organization | (24)4 silencing | (38)2 clusters | (52)2 synteny |
(11)7 sequences | (25)4 to | (39)2 content | (53)2 therapy, |
(12)5 flow | (26)4 which | (40)2 family | (54)2 used |
(13)5 for | (27)3 alterations | (41)2 from | (55)2 uses |
(14)5 in | (28)3 as | (42)2 has |
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