ELIZA

return kwic search for human out of >500 occurrences
482327 occurrences (No.32 in the rank) during 5 years in the PubMed. [no cache] 500 found

95) Human umbilical vessels have been recognized as a valuable and widely available resource for vascular tissue engineering.

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PMID:23038628 DOI:10.1002/term.1603

2015 Journal of tissue engineering and regenerative medicine

* Evaluation of decellularized human umbilical vein (HUV) for vascular tissue engineering - comparison with endothelium-denuded HUV.

- Human umbilical vessels have been recognized as a valuable and widely available resource for vascular tissue engineering. Whereas endothelium-denuded human umbilical veins (HUVs) have been successfully seeded with a patient-derived neoendothelium, decellularized vessels may have additional advantages, due to their lower antigenicity. The present study investigated the effects of three different decellularization procedures on the histological, mechanical and seeding properties of HUVs. Vessels were decellularized by detergent treatment (Triton X-100, sodium deoxycholate, IGEPAL-CA630), osmotic lysis (3 m NaCl, distilled water) and peroxyacetic acid treatment. In all cases, nuclease treatments were required to remove residual nucleic acids. Decellularization resulted in a partial loss of fibronectin and laminin staining in the subendothelial layer and affected the appearance of elastic fibres. In addition to removing residual nucleic acids, nuclease treatment weakened all stainings and substantially altered surface properties, as seen in scanning electron micrographs, indicating additional non-specific effects. Detergent treatment and osmotic lysis caused failure stresses to decrease significantly. Although conditioned medium prepared from decellularized HUV did not severely affect endothelial cell growth, cells seeded on decellularized HUV did not remain viable. This may be attributed to the partial removal of essential extracellular matrix components as well as to changes of surface properties. Therefore, decellularized HUVs appear to require additional modifications in order to support successful cell seeding. Replacing the vessels' endothelium may thus be a superior alternative to decellularization when creating tissue-engineered blood vessels with non-immunogenic luminal interfaces.

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[frequency of next (right) word to human]

(1)19 and	(23)3 blood	(45)2 adipose-derived	(67)2 insulin
(2)15 health	(24)3 bronchial	(46)2 amniotic	(68)2 interaction
(3)11 brain	(25)3 dental	(47)2 articular	(69)2 islet
(4)10 breast	(26)3 dermal	(48)2 body,	(70)2 leukocyte
(5)8 body	(27)3 embryonic	(49)2 brain,	(71)2 liver
(6)8 mesenchymal	(28)3 head	(50)2 but	(72)2 microbiota
(7)8 papillomavirus	(29)3 lung	(51)2 cartilage	(73)2 mycetoma
(8)7 bone	(30)3 nasal	(52)2 cerebral	(74)2 neuroblastoma
(9)7 subjects	(31)3 renal	(53)2 chorionic	(75)2 oral
(10)7 umbilical	(32)3 serum	(54)2 consumption,	(76)2 osteosarcoma
(11)6 cancer	(33)3 skin	(55)2 cortical	(77)2 papilloma
(12)6 hepatocytes	(34)3 spinal	(56)2 disease	(78)2 participants
(13)4 null	(35)3 teeth	(57)2 endometrial	(79)2 periodontal
(14)4 MSCs	(36)3 tissues	(58)2 endothelial	(80)2 platelet
(15)4 cancers	(37)2 Alu	(59)2 eye	(81)2 primary
(16)4 cancers,	(38)2 CD34(+)	(60)2 face	(82)2 resources
(17)4 immunodeficiency	(39)2 DMN	(61)2 fibroblasts	(83)2 respiratory
(18)4 model	(40)2 PIF1	(62)2 glioma	(84)2 rights
(19)4 oncology	(41)2 TP	(63)2 health,	(85)2 studies
(20)4 plasma	(42)2 abdomen	(64)2 hepatocellular	(86)2 third
(21)3 Province	(43)2 activity	(65)2 induced	(87)2 tooth
(22)3 acellular	(44)2 adipose	(66)2 infections,

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--- WordNet output for human --- =>人間らしい, 人間の, 人, 人間 Overview of noun human The noun human has 1 sense (first 1 from tagged texts) 1. (5) homo, man, human being, human -- (any living or extinct member of the family Hominidae characterized by superior intelligence, articulate speech, and erect carriage) Overview of adj human The adj human has 3 senses (first 3 from tagged texts) 1. (46) human -- (characteristic of humanity; "human nature") 2. (19) human -- (relating to a person; "the experiment was conducted on 6 monkeys and 2 human subjects") 3. (15) human -- (having human form or attributes as opposed to those of animals or divine beings; "human beings"; "the human body"; "human kindness"; "human frailty") --- WordNet end ---