* Analysis of imprinted gene expression and implantation in haploid androgenetic mouse embryos.
- The successful development of mammalian embryos requires both parental genomes. Nuclear transfer techniques have been adapted to generate uniparental embryos, which possess two sets of paternal or maternal genomes. These embryos fail to develop to term because of abnormal imprinted gene expression, which is not regulated by Mendelian inheritance. Uniparental embryos provide us with an important model to investigate imprinted gene function and ontogenesis. To evaluate the pre- and post-developmental ability of haploid androgenetic mouse embryos, and to analyse the expression of imprinted genes Igf2r, Asb4 and Mest in haploid androgenetic/gynogenetic blastocysts, we produced the haploid mouse embryos using the enucleation technique, examined their development at 6.5 dpc and quantified gene expression by quantitative real-time PCR. The results demonstrated that the developmental potential of haploid embryos was severely impaired and revealed that the haploid androgenones could induce the deciduas reaction, but failed to retain a live foetus at 6.5 dpc. Expression of imprinted genes Igf2r and Asb4 was unregulated in haploid androgenetic/gynogenetic blastocysts.
=>重要な, 有力な, 重大な, 大切な
Overview of adj important
The adj important has 5 senses (first 4 from tagged texts)
1. (146) important, of import -- (of great significance or value; "important people"; "the important
questions of the day")
2. (4) significant, important -- (important in effect or meaning; "a significant change in tax
laws"; "a significant change in the Constitution"; "a significant contribution"; "significant
details"; "statistically significant")
3. (2) crucial, important -- (of extreme importance; vital to the resolution of a crisis; "a crucial
moment in his career"; "a crucial election"; "a crucial issue for women")
4. (1) authoritative, important -- (having authority or ascendancy or influence; "an important
official"; "the captain's authoritative manner")
5. important -- (having or suggesting a consciousness of high position; "recited the decree with an
important air"; "took long important strides in the direction of his office")
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