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448856 occurrences (No.37 in the rank) during 5 years in the PubMed. [cache]
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448856 occurrences (No.37 in the rank) during 5 years in the PubMed. [cache]
| 88) Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. |
| PMID:24388586 DOI:10.1016/j.jmii.2013.09.010 |
| 2015 Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi |
| * Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus. |
| - Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies. |
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