ELIZA cgi-bash version rev. 1.90
- Medical English LInking keywords finder for the PubMed Zipped Archive (ELIZA) -

return kwic search for increased out of >500 occurrences
611425 occurrences (No.13 in the rank) during 5 years in the PubMed. [no cache] 500 found
21) Drug treatment either throughout IVP or only during IVF decreased the percentage of ≥ 8-cell embryos (1 μM, P < 0.05; 10 μM, P < 0.01; and 0.1 μM, 10 μM, P < 0.01, respectively) and increased the percentage of 2-cell embryos (10 μM, P < 0.01 and P < 0.05 respectively).
--- ABSTRACT ---
PMID:23870192 DOI:10.1017/S0967199413000300
2015 Zygote (Cambridge, England)
* Effects of metformin on fertilisation of bovine oocytes and early embryo development: possible involvement of AMPK3-mediated TSC2 activation.
- Studies on bovine oocytes have revealed that the activation of adenosine monophosphate activated protein kinase (AMPK) by millimolar concentrations of metformin controls nuclear maturation. Tuberous sclerosis complex 2 (TSC2) has been identified as a downstream target of AMPK. The objective of this study was to investigate the effects of addition of low concentrations of metformin (1 nM to 10 μM) on the percentage of cultured cumulus-oocyte complexes (COC) giving rise to cleavage-stage embryos and AMPK-mediated TSC2 activation. Metformin was supplemented either throughout in vitro embryo production (IVP) or only during in vitro fertilization (IVF). COC were matured in vitro, inseminated, and presumptive zygotes cultured for a further 72 h post insemination before the percentage of COC that gave rise to zygotes and early embryo development was assessed. The presence of TSC2 in bovine embryos and its possible AMPK-induced activation were assessed by immunocytochemistry. Metformin had a dose-dependent effect on the numbers of cultured COC that gave rise to embryos. Drug treatment either throughout IVP or only during IVF decreased the percentage of ≥ 8-cell embryos (1 μM, P < 0.05; 10 μM, P < 0.01; and 0.1 μM, 10 μM, P < 0.01, respectively) and increased the percentage of 2-cell embryos (10 μM, P < 0.01 and P < 0.05 respectively). The percentage of cultured COC that gave rise to zygotes was not affected by metformin. TSC2 is expressed in early embryos. Metformin (10 μM) either throughout IVP or during IVF only, increased AMPK-induced PhosphoS1387-TSC2 immunoreactivity (P < 0.01) and this increase corresponded to the total TSC2 protein levels expressed in cells. Our results suggest that there is a dose-dependent negative effect of metformin on the ability of oocytes to cleave following insemination, possibly mediated through an AMPK-induced activation of TSC2.
--- ABSTRACT END ---
[
right
kwic]
[frequency of next (right) word to increased]
(1)36 the (14)5 to (27)2 RDW (40)2 likelihood
(2)34 in (15)4 after (28)2 awareness (41)2 maternal
(3)27 risk (16)4 as (29)2 bone (42)2 morbidity
(4)21 *null* (17)3 activity (30)2 but (43)2 number
(5)20 with (18)3 among (31)2 compared (44)2 over
(6)16 by (19)3 at (32)2 concentrations (45)2 pre-stimulus
(7)15 significantly (20)3 cell (33)2 drug (46)2 production
(8)6 and (21)3 level (34)2 during (47)2 rapidly
(9)6 expression (22)3 levels (35)2 fax (48)2 use
(10)6 from (23)3 physical (36)2 functional (49)2 vascular
(11)5 activation (24)3 sensitivity (37)2 hepatic
(12)5 odds (25)2 MI (38)2 if
(13)5 serum (26)2 Math1 (39)2 interest

add keyword

--- WordNet output for increased --- =>力の増加 Overview of verb increase The verb increase has 2 senses (first 2 from tagged texts) 1. (86) increase -- (become bigger or greater in amount; "The amount of work increased") 2. (61) increase -- (make bigger or more; "The boss finally increased her salary"; "The university increased the number of students it admitted") Overview of adj increased The adj increased has 1 sense (first 1 from tagged texts) 1. (30) increased -- (made greater in size or amount or degree) --- WordNet end ---