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return kwic search for novel out of >500 occurrences
330250 occurrences (No.59 in the rank) during 5 years in the PubMed. [no cache] 500 found

75) Here we describe a novel method for in situ detection of IsoLGs in tissues using alkaline phosphatase-conjugated D11 ScFv and a recombinant phage-display antibody produced in E.

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PMID:34279509 DOI:10.3791/62603

2021 Journal of visualized experiments : JoVE

* Direct Detection of Isolevuglandins in Tissues using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence.

- Isolevuglandins (IsoLGs) are highly reactive gamma ketoaldehydes formed from H2-isoprostanes through lipid peroxidation and crosslink proteins leading to inflammation and various diseases including hypertension. Detection of IsoLG accumulation in tissues is crucial in shedding light on their involvement in the disease processes. However, measurement of IsoLGs in tissues is extremely difficult, and currently available tools, including mass spectrometry analysis, are laborious and extremely expensive. Here we describe a novel method for in situ detection of IsoLGs in tissues using alkaline phosphatase-conjugated D11 ScFv and a recombinant phage-display antibody produced in E. coli by immunofluorescent microscopy. Four controls were used for validating the staining: (1) staining with and without D11, (2) staining with bacterial periplasmic extract with the alkaline phosphatase linker, (3) irrelevant scFV antibody staining, and (4) competitive control with IsoLG prior to the staining. We demonstrate the effectiveness of the alkaline phosphatase-conjugated D11 in both human and mouse tissues with or without hypertension. This method will likely serve as an important tool to study the role of IsoLGs in a wide variety of disease processes.

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(1)23 therapeutic	(18)4 strategy	(35)2 antifungal	(52)2 nonsteroidal
(2)14 approach	(19)4 therapeutics	(36)2 biomarker	(53)2 object
(3)11 and	(20)3 STAT3	(37)2 biomarkers	(54)2 opportunities
(4)10 coronavirus	(21)3 avenues	(38)2 blood	(55)2 perspectives
(5)8 insights	(22)3 bicistronic	(39)2 cardiac	(56)2 pharmaceutical
(6)8 mechanism	(23)3 compound	(40)2 cell-based	(57)2 preclinical
(7)7 method	(24)3 diagnostic	(41)2 class	(58)2 protein
(8)6 chemical	(25)3 drugs	(42)2 dietary	(59)2 proteins
(9)6 role	(26)3 genetic	(43)2 evidence-based	(60)2 reactive
(10)6 therapies	(27)3 immunotherapeutic	(44)2 human	(61)2 risk
(11)6 treatment	(28)3 methodologies	(45)2 imaging	(62)2 small-molecule
(12)5 approaches	(29)3 mouse	(46)2 infectious	(63)2 target
(13)5 drug	(30)3 small	(47)2 insight	(64)2 targets
(14)4 data	(31)2 Coronavirus	(48)2 interventions	(65)2 trial
(15)4 findings	(32)2 agents	(49)2 methodology
(16)4 in	(33)2 analgesic	(50)2 neuroprotective
(17)4 strategies	(34)2 analytical	(51)2 noninvasive

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--- WordNet output for novel --- =>1.よく知られていない, 新しい, 珍奇な, 異常な, 新奇な, 目新しい, 2.(長編)小説 Overview of noun novel The noun novel has 2 senses (first 1 from tagged texts) 1. (17) novel -- (an extended fictional work in prose; usually in the form of a story) 2. novel -- (a printed and bound book that is an extended work of fiction; "his bookcases were filled with nothing but novels"; "he burned all the novels") Overview of adj novel The adj novel has 2 senses (first 1 from tagged texts) 1. (6) fresh, new, novel -- (original and of a kind not seen before; "the computer produced a completely novel proof of a well-known theorem") 2. novel, refreshing -- (pleasantly new or different; "common sense of a most refreshing sort") --- WordNet end ---