257) Several studies specifically addressed the potential association between a recent vaccination and BTV-8 RNA detection in the blood of sheep. |
PMID:23611408 DOI:10.1111/tbed.12100 |
2015 Transboundary and emerging diseases |
* Bluetongue virus RNA detection by real-time rt-PCR in post-vaccination samples from cattle. |
- Bluetongue virus serotype 8 (BTV-8) was responsible for a large outbreak among European ruminant populations in 2006-2009. In spring 2008, a massive vaccination campaign was undertaken, leading to the progressive disappearance of the virus. During surveillance programmes in Western Europe in 2010-2011, a low but significant number of animals were found weakly positive using BTV-specific real-time RT-PCR, raising questions about a possible low level of virus circulation. An interference of the BTV-8 inactivated vaccine on the result of the real-time RT-PCR was also hypothesized. Several studies specifically addressed the potential association between a recent vaccination and BTV-8 RNA detection in the blood of sheep. Results were contradictory and cattles were not investigated. To enlighten this point, a large study was performed to determine the risks of detection of bluetongue vaccine-associated RNA in the blood and spleen of cattle using real-time RT-PCR. Overall, the results presented clearly demonstrate that vaccine viral RNA can reach the blood circulation in sufficient amounts to be detected by real-time RT-PCR in cattle. This BTV-8 vaccine RNA carriage appears as short lasting. |
(1)67 of | (15)4 effects | (29)2 by | (43)2 mediator |
(2)39 to | (16)4 guardian | (30)2 clinical | (44)2 new |
(3)35 for | (17)4 mechanism | (31)2 confounders | (45)2 pathways |
(4)14 *null* | (18)4 mechanisms | (32)2 confounders, | (46)2 perils |
(5)13 in | (19)3 adverse | (33)2 directions | (47)2 preventive |
(6)10 as | (20)3 applications | (34)2 drug | (48)2 risks |
(7)10 risk | (21)3 association | (35)2 factors | (49)2 than |
(8)8 role | (22)3 cytotoxicity | (36)2 future | (50)2 therapy |
(9)8 use | (23)3 genotoxic | (37)2 harm | (51)2 toxicity |
(10)7 and | (24)3 influence | (38)2 hazards | (52)2 vaccine |
(11)7 therapeutic | (25)3 pitfalls | (39)2 impact | (53)2 was |
(12)6 benefits | (26)3 utility | (40)2 is | |
(13)5 health | (27)3 value | (41)2 issues | |
(14)5 predictors | (28)2 biomarker | (42)2 maximum |
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