ELIZA

return kwic search for protein out of >500 occurrences
536761 occurrences (No.22 in the rank) during 5 years in the PubMed. [no cache] 500 found

223) Stabilisation of HIF1α protein in cumulus cells exposed to low oxygen was confirmed by western blot and HIF-mediated transcriptional activity was demonstrated using a transgenic mouse expressing green fluorescent protein under the control of a promoter containing hypoxia response elements.

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PMID:24388334 DOI:10.1071/RD13249

2015 Reproduction, fertility, and development

* Oxygen-regulated gene expression in murine cumulus cells.

- Oxygen is an important component of the environment of the cumulus-oocyte complex (COC), both in vivo within the ovarian follicle and during in vitro oocyte maturation (IVM). Cumulus cells have a key role in supporting oocyte development, and cumulus cell function and gene expression are known to be altered when the environment of the COC is perturbed. Oxygen-regulated gene expression is mediated through the actions of the transcription factors, the hypoxia-inducible factors (HIFs). In the present study, the effect of oxygen on cumulus cell gene expression was examined following in vitro maturation of the murine COC at 2%, 5% or 20% oxygen. Increased expression of HIF-responsive genes, including glucose transporter-1, lactate dehydrogenase A and BCL2/adenovirus E1B interacting protein 3, was observed in cumulus cells matured at 2% or 5%, compared with 20% oxygen. Stabilisation of HIF1α protein in cumulus cells exposed to low oxygen was confirmed by western blot and HIF-mediated transcriptional activity was demonstrated using a transgenic mouse expressing green fluorescent protein under the control of a promoter containing hypoxia response elements. These results indicate that oxygen concentration influences cumulus cell gene expression and support a role for HIF1α in mediating the cumulus cell response to varying oxygen.

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(1)76 genes,	(11)8 was	(21)3 has	(31)2 for
(2)45 genes	(12)7 level	(22)3 is	(32)2 initiation
(3)24 and	(13)6 carbonyl	(23)3 of	(33)2 level,
(4)23 levels	(14)6 coding	(24)3 oxidation,	(34)2 production
(5)18 expression	(15)5 C	(25)3 stability	(35)2 sequences
(6)12 null	(16)5 gene	(26)3 thiol	(36)2 solutions
(7)12 kinase	(17)5 isolate	(27)2 acid	(37)2 structures
(8)9 1	(18)5 kinases	(28)2 content	(38)2 that
(9)9 in	(19)4 levels,	(29)2 contents
(10)8 2	(20)3 expression,	(30)2 families

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--- WordNet output for protein --- =>タンパク質, 蛋白質, たん白質 Overview of noun protein The noun protein has 1 sense (first 1 from tagged texts) 1. (23) protein -- (any of a large group of nitrogenous organic compounds that are essential constituents of living cells; consist of polymers of amino acids; essential in the diet of animals for growth and for repair of tissues; can be obtained from meat and eggs and milk and legumes; "a diet high in protein") --- WordNet end ---