ELIZA

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536761 occurrences (No.22 in the rank) during 5 years in the PubMed. [no cache] 500 found

228) Here we report the (1)H, (13)C and (15)N chemical shift assignments, secondary structure and chemical shift deviations from random coil of the domain of human heparanase comprising residues Lys158-Lys417, a construct that has been validated as surrogate of the full length protein in the search of novel inhibitors for this enzyme.

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PMID:24395156 DOI:10.1007/s12104-013-9536-9

2015 Biomolecular NMR assignments

* Chemical shift assignments and secondary structure of the surrogate domain for drug discovery studies of human heparanase.

- Heparanase is an endoglycosidase that specifically degrades heparan sulfate, one of the main components of the extracellular matrix. Heparanase is implicated in cancer processes such as tumour formation, angiogenesis and metastasis, making it a very attractive target in drug discovery. Its active form is a heterodimer constituted by a 45 kDa glycosylated subunit (Lys158-Ile543) non-covalently bound to a smaller 8 kDa polypeptide (Gln36-Glu109). Residues Glu225 and Glu343 are critical in its catalytic mechanism and two heparan sulfate binding sites (Lys158-Asp171 and Gln270-Lys280) have been identified in the enzyme. Here we report the (1)H, (13)C and (15)N chemical shift assignments, secondary structure and chemical shift deviations from random coil of the domain of human heparanase comprising residues Lys158-Lys417, a construct that has been validated as surrogate of the full length protein in the search of novel inhibitors for this enzyme.

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(1)76 genes,	(11)8 was	(21)3 has	(31)2 for
(2)45 genes	(12)7 level	(22)3 is	(32)2 initiation
(3)24 and	(13)6 carbonyl	(23)3 of	(33)2 level,
(4)23 levels	(14)6 coding	(24)3 oxidation,	(34)2 production
(5)18 expression	(15)5 C	(25)3 stability	(35)2 sequences
(6)12 null	(16)5 gene	(26)3 thiol	(36)2 solutions
(7)12 kinase	(17)5 isolate	(27)2 acid	(37)2 structures
(8)9 1	(18)5 kinases	(28)2 content	(38)2 that
(9)9 in	(19)4 levels,	(29)2 contents
(10)8 2	(20)3 expression,	(30)2 families

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--- WordNet output for protein --- =>タンパク質, 蛋白質, たん白質 Overview of noun protein The noun protein has 1 sense (first 1 from tagged texts) 1. (23) protein -- (any of a large group of nitrogenous organic compounds that are essential constituents of living cells; consist of polymers of amino acids; essential in the diet of animals for growth and for repair of tissues; can be obtained from meat and eggs and milk and legumes; "a diet high in protein") --- WordNet end ---