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280501 occurrences (No.89 in the rank) during 5 years in the PubMed. [no cache] 500 found

126) Expanding undifferentiated induced pluripotent stem (iPS) cells in vitro is a basic requirement for application of iPS cells in both fundamental research and clinical regeneration.

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PMID:23166055 DOI:10.1002/term.1640

2015 Journal of tissue engineering and regenerative medicine

* Hydrogels as feeder-free scaffolds for long-term self-renewal of mouse induced pluripotent stem cells.

- Expanding undifferentiated induced pluripotent stem (iPS) cells in vitro is a basic requirement for application of iPS cells in both fundamental research and clinical regeneration. In this study, we intended to establish a simple, low cost and efficient method for the long-term self-renewal of mouse induced pluripotent stem (miPS) cells without using feeder-cells and adhesive proteins. Three scaffolds were selected for the long-term subculture of miPS cells over two months starting from passages 14 to 29: 1) a gelatin coated polystyrene (Gelatin-PS) that is a widely used scaffold for self-renewal of mouse embryonic stem (mES) cells; 2) a neutral hydrogel poly(N,N-dimethylacrylamide) (PDMAAm); and 3) a negatively charged hydrogel poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS). Each passaged miPS cells on these scaffolds were cryopreserved successfully and the revived cells showed high viability and proliferation. The passaged miPS cells maintained a high undifferentiated state on all three scaffolds and a high level of pluripotency by expressing differentiation markers in vitro and forming teratomas in SCID mice with derivatives of all three germ layers. Compared to Gelatin-PS, the two hydrogels exhibited much better self-renewal performance in terms of high proliferation rate and level of expression of undifferentiated gene markers as well as efficiency in pluripotent teratoma formation. Furthermore, the PNaAMPS hydrogel demonstrated a slightly higher efficiency and simpler operation of cell expansion than the PDMAAm hydrogel. To conclude, PNaAMPS hydrogel is an excellent feeder-free scaffold because of its simplicity, low cost and high efficiency in expanding a large number of miPS cells in vitro.

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(2)40 on	(15)6 by	(28)3 directions	(41)2 involving
(3)36 is	(16)6 to	(29)3 evidence	(42)2 participants,
(4)27 and	(17)5 projects	(30)3 have	(43)2 progress
(5)25 has	(18)4 culture	(31)3 method	(44)2 sponsored
(6)14 in	(19)4 design	(32)3 results	(45)2 the
(7)12 was	(20)4 findings	(33)3 shows	(46)2 topic
(8)11 are	(21)4 needs	(34)3 study	(47)2 towards
(9)11 should	(22)4 project	(35)3 will	(48)2 trends
(10)9 into	(23)4 questions	(36)2 efforts	(49)2 using
(11)9 with	(24)4 regarding	(37)2 examined	(50)2 we
(12)8 studies	(25)3 &	(38)2 focus	(51)2 work
(13)8 suggests	(26)3 aimed	(39)2 for

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--- WordNet output for research --- =>調査する, 研究(する), 探求, 調査, 捜索, 研究する Overview of noun research The noun research has 2 senses (first 2 from tagged texts) 1. (46) research -- (systematic investigation to establish facts) 2. (6) inquiry, enquiry, research -- (a search for knowledge; "their pottery deserves more research than it has received") Overview of verb research The verb research has 2 senses (first 1 from tagged texts) 1. (1) research -- (attempt to find out in a systematically and scientific manner; "The student researched the history of that word") 2. research, search, explore -- (inquire into; "the students had to research the history of the Second World War for their history project"; "He searched for information on his relatives on the web"; "Scientists are exploring the nature of consciousness") --- WordNet end ---