280) Genotoxicity assessment revealed no increase in the number of micronucleated binucleated cells and no induction of SBs or oxidised DNA bases in PLGA-PEO-treated cells. |
PMID:23859252 DOI:10.3109/17435390.2013.816798 |
2015 Nanotoxicology |
* Immunotoxicity and genotoxicity testing of PLGA-PEO nanoparticles in human blood cell model. |
- A human blood cell model for immunotoxicity and genotoxicity testing was used to measure the response to polylactic-co-glycolic acid (PLGA-PEO) nanoparticle (NP) (0.12, 3, 15 and 75 μg/cm(2) exposure in fresh peripheral whole blood cultures/isolated peripheral blood mononuclear cell cultures from human volunteers (n = 9-13). PLGA-PEO NPs were not toxic up to dose 3 μg/cm(2); dose of 75 μg/cm(2) displays significant decrease in [(3)H]-thymidine incorporation into DNA of proliferating cells after 4 h (70% of control) and 48 h (84%) exposure to NPs. In non-cytotoxic concentrations, in vitro assessment of the immunotoxic effects displayed moderate but significant suppression of proliferative activity of T-lymphocytes and T-dependent B-cell response in cultures stimulated with PWM > CON A, and no changes in PHA cultures. Decrease in proliferative function was the most significant in T-cells stimulated with CD3 antigen (up to 84%). Cytotoxicity of natural killer cells was suppressed moderately (92%) but significantly in middle-dosed cultures (4 h exposure). On the other hand, in low PLGA-PEO NPs dosed cultures, significant stimulation of phagocytic activity of granulocytes (119%) > monocytes (117%) and respiratory burst of phagocytes (122%) was recorded. Genotoxicity assessment revealed no increase in the number of micronucleated binucleated cells and no induction of SBs or oxidised DNA bases in PLGA-PEO-treated cells. To conclude on immuno- and genotoxicity of PLGA-PEO NPs, more experiments with various particle size, charge and composition need to be done. |
(1)193 that | (9)4 five | (17)2 common | (25)2 new |
(2)61 a | (10)4 high | (18)2 distinct | (26)2 numerous |
(3)21 the | (11)3 statistically | (19)2 enhanced | (27)2 right |
(4)14 no | (12)3 that, | (20)2 evidence | (28)2 severe |
(5)13 an | (13)3 three | (21)2 four | (29)2 significantly |
(6)13 significant | (14)3 two | (22)2 higher | |
(7)7 by | (15)2 *null* | (23)2 in | |
(8)5 multiple | (16)2 as | (24)2 moderate |
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