ELIZA

return kwic search for significantly out of >500 occurrences
583352 occurrences (No.14 in the rank) during 5 years in the PubMed. [cache]

90) 2% O(2) resulted in a significantly lower CD34(+) cell expansion (25-fold vs 60-, 64- and 92-fold at day 10 for 5%, 21%, 10% O(2), respectively).

--- ABSTRACT ---

PMID:23596131 DOI:10.1002/term.1731

2015 Journal of tissue engineering and regenerative medicine

* Ex vivo expansion of cord blood haematopoietic stem/progenitor cells under physiological oxygen tensions: clear-cut effects on cell proliferation, differentiation and metabolism.

- Physiologically low O(2) tensions are believed to regulate haematopoietic stem cell (HSC) functions in the bone marrow (BM; 0-5%). In turn, placenta and umbilical cord are characterized by slightly higher physiological O(2) tensions (3-10%). We hypothesized that O(2) concentrations within this range may be exploited to augment the ex vivo expansion/maintenance of HSCs from umbilical cord (placental) blood (UCB). The expansion of UCB CD34(+) -enriched cells was studied in co-culture with BM mesenchymal stem/stromal cells (MSCs) under 2%, 5%, 10% and 21% O(2). 2% O(2) resulted in a significantly lower CD34(+) cell expansion (25-fold vs 60-, 64- and 92-fold at day 10 for 5%, 21%, 10% O(2), respectively). In turn, 10% O(2) promoted the highest CD34(+) CD90(+) cell expansion, reaching 22 ± 5.4- vs 5.6 ± 2.4- and 5.7 ± 2.0-fold for 2%, 5% and 21% O(2), respectively, after 14 days. Similar differentiation patterns were observed under different O(2) tensions, being primarily shifted towards the neutrophil lineage. Cell division kinetics revealed a higher proliferative status of cells cultured under 10% and 21% vs 2% O(2). Expectedly, higher specific glucose consumption and lactate production rates were determined at 2% O(2) when compared to higher O(2) concentrations (5-21%). Overall, these results suggest that physiological oxygen tensions, in particular 10% O(2), can maximize the ex vivo expansion of UCB stem/progenitor cells in co-culture with BM MSCs. Importantly, these studies highlight the importance of exploiting knowledge of the intricate microenvironment of the haematopoietic niche towards the definition of efficient and controlled ex vivo culture systems capable of generating large HSCs numbers for clinical applications.

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[frequency of next (right) word to significantly]

(1)88 higher	(13)8 improved	(25)3 (P<0.05)	(39)2 impaired
(2)43 lower	(14)6 correlated	(27)3 affect	(40)2 increases
(3)39 increased	(15)6 elevated	(28)3 better	(41)2 induced
(4)35 reduced	(16)6 fewer	(29)3 increase	(42)2 larger
(5)32 associated	(17)6 less	(30)3 worse	(43)2 predicted
(6)17 decreased	(18)5 enhanced	(31)2 (P	(44)2 reduce
(7)13 in	(19)5 inhibited	(32)2 (p	(45)2 shorter
(8)13 more	(20)4 affected	(34)2 altered	(46)2 smaller
(9)10 greater	(21)4 attenuated	(35)2 by	(47)2 suppressed
(10)10 related	(22)4 between	(36)2 compared	(48)2 to
(11)9 null	(23)4 from	(37)2 decreased,
(12)9 different	(24)4 the	(38)2 differed

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--- WordNet output for significantly --- =>きわめて, 意味深く, 意味ありげに Overview of adv significantly The adv significantly has 3 senses (first 2 from tagged texts) 1. (6) significantly -- (in a statistically significant way; "the two groups differed significantly") 2. (3) significantly -- (in a significant manner; "our budget will be significantly affected by these new cuts") 3. importantly, significantly -- (in an important way or to an important degree; "more importantly, Weber held that the manifold meaning attached to the event by the social scientist could alter his definition of the concrete event itself") --- WordNet end ---