ELIZA

return kwic search for specific out of >500 occurrences
279736 occurrences (No.90 in the rank) during 5 years in the PubMed. [cache]

84) In addition to removing residual nucleic acids, nuclease treatment weakened all stainings and substantially altered surface properties, as seen in scanning electron micrographs, indicating additional non-specific effects.

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PMID:23038628 DOI:10.1002/term.1603

2015 Journal of tissue engineering and regenerative medicine

* Evaluation of decellularized human umbilical vein (HUV) for vascular tissue engineering - comparison with endothelium-denuded HUV.

- Human umbilical vessels have been recognized as a valuable and widely available resource for vascular tissue engineering. Whereas endothelium-denuded human umbilical veins (HUVs) have been successfully seeded with a patient-derived neoendothelium, decellularized vessels may have additional advantages, due to their lower antigenicity. The present study investigated the effects of three different decellularization procedures on the histological, mechanical and seeding properties of HUVs. Vessels were decellularized by detergent treatment (Triton X-100, sodium deoxycholate, IGEPAL-CA630), osmotic lysis (3 m NaCl, distilled water) and peroxyacetic acid treatment. In all cases, nuclease treatments were required to remove residual nucleic acids. Decellularization resulted in a partial loss of fibronectin and laminin staining in the subendothelial layer and affected the appearance of elastic fibres. In addition to removing residual nucleic acids, nuclease treatment weakened all stainings and substantially altered surface properties, as seen in scanning electron micrographs, indicating additional non-specific effects. Detergent treatment and osmotic lysis caused failure stresses to decrease significantly. Although conditioned medium prepared from decellularized HUV did not severely affect endothelial cell growth, cells seeded on decellularized HUV did not remain viable. This may be attributed to the partial removal of essential extracellular matrix components as well as to changes of surface properties. Therefore, decellularized HUVs appear to require additional modifications in order to support successful cell seeding. Replacing the vessels' endothelium may thus be a superior alternative to decellularization when creating tissue-engineered blood vessels with non-immunogenic luminal interfaces.

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(1)15 to	(19)4 types	(37)2 attention	(55)2 lineages
(2)9 sequence	(20)3 characteristics	(38)2 barriers	(56)2 measures
(3)7 and	(21)3 features	(39)2 computational	(57)2 miRNAs
(4)7 cell	(22)3 job	(40)2 constitutive	(58)2 molecular
(5)7 variations	(23)3 target	(41)2 criteria	(59)2 neuronal
(6)6 cognitive	(24)3 tendon	(42)2 deficits	(60)2 or
(7)6 genes	(25)3 therapy	(43)2 differences	(61)2 parameters
(8)6 surface	(26)3 type	(44)2 divergence	(62)2 phytotoxicity
(9)5 antibodies	(27)3 variation	(45)2 effect	(63)2 polymerase
(10)5 for	(28)2 null	(46)2 elements	(64)2 primers
(11)5 phobias	(29)2 ECM	(47)2 ethnocultural	(65)2 protein
(12)4 brain	(30)2 IgG	(48)2 extracellular	(66)2 proteins
(13)4 effects	(31)2 PCR	(49)2 fault	(67)2 recommendations
(14)4 growth	(32)2 age-dependent	(50)2 function	(68)2 requirements
(15)4 incidence	(33)2 analysis	(51)2 genetic	(69)2 self-efficacy
(16)4 manner	(34)2 antigen	(52)2 genomic	(70)2 targeted
(17)4 motor	(35)2 antigens	(53)2 information	(71)2 tests
(18)4 needs	(36)2 approach	(54)2 inhibitors

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--- WordNet output for specific --- =>特有の, 特効薬, 明確な, 特定の, 種の, はっきりした Overview of noun specific The noun specific has 2 senses (no senses from tagged texts) 1. particular, specific -- (a fact about some part (as opposed to general); "he always reasons from the particular to the general") 2. specific -- (a medicine that has a mitigating effect on a specific disease; "quinine is a specific for malaria") Overview of adj specific The adj specific has 4 senses (first 2 from tagged texts) 1. (39) specific -- ((sometimes followed by `to') applying to or characterized by or distinguishing something particular or special or unique; "rules with specific application"; "demands specific to the job"; "a specific and detailed account of the accident") 2. (5) specific -- (stated explicitly or in detail; "needed a specific amount") 3. specific -- (relating to or distinguishing or constituting a taxonomic species; "specific characters") 4. specific -- (being or affecting a disease produced by a particular microorganism or condition; used also of stains or dyes used in making microscope slides; "quinine is highly specific for malaria"; "a specific remedy"; "a specific stain is one having a specific affinity for particular structural elements") --- WordNet end ---