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532339 occurrences (No.23 in the rank) during 5 years in the PubMed. [no cache] 500 found

203) Future studies on the mechanisms of attenuation of oxidative injury by NQO1 should help in developing novel approaches for the prevention/treatment of ARDS in humans.

--- ABSTRACT ---

PMID:34691356 DOI:10.1155/2021/5544600

2021 Oxidative medicine and cellular longevity

* Role of Human NADPH Quinone Oxidoreductase (NQO1) in Oxygen-Mediated Cellular Injury and Oxidative DNA Damage in Human Pulmonary Cells.

- Supplemental oxygen administration is frequently used in premature infants and adults with pulmonary insufficiency. NADPH quinone oxidoreductase (NQO1) protects cells from oxidative injury by decreasing reactive oxygen species (ROS). In this investigation, we tested the hypothesis that overexpression of NQO1 in BEAS-2B cells will mitigate cell injury and oxidative DNA damage caused by hyperoxia and that A-1221C single nucleotide polymorphism (SNP) in the NQO1 promoter would display altered susceptibility to hyperoxia-mediated toxicity. Using stable transfected BEAS-2B cells, we demonstrated that hyperoxia decreased cell viability in control cells (Ctr), but this effect was differentially mitigated in cells overexpressing NQO1 under the regulation of the CMV viral promoter, the wild-type NQO1 promoter (NQO1-NQO1), or the NQO1 promoter carrying the SNP. Interestingly, hyperoxia decreased the formation of bulky oxidative DNA adducts or 8-hydroxy-2'-deoxyguanosine (8-OHdG) in Ctr cells. qPCR studies showed that mRNA levels of CYP1A1 and NQO1 were inversely related to DNA adduct formation, suggesting the protective role of these enzymes against oxidative DNA injury. In SiRNA experiments entailing the NQO1-NQO1 promoter, hyperoxia caused decreased cell viability, and this effect was potentiated in cells treated with CYP1A1 siRNA. We also found that hyperoxia caused a marked induction of DNA repair genes DDB2 and XPC in Ctr cells, supporting the idea that hyperoxia in part caused attenuation of bulky oxidative DNA lesions by enhancing nucleotide excision repair (NER) pathways. In summary, our data support a protective role for human NQO1 against oxygen-mediated toxicity and oxidative DNA lesions in human pulmonary cells, and protection against toxicity was partially lost in SNP cells. Moreover, we also demonstrate a novel protective role for CYP1A1 in the attenuation of oxidative cells and DNA injury. Future studies on the mechanisms of attenuation of oxidative injury by NQO1 should help in developing novel approaches for the prevention/treatment of ARDS in humans.

--- ABSTRACT END ---

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(1)44 null	(15)7 should	(29)4 showed	(43)2 had
(2)35 have	(16)7 suggest	(30)4 the	(44)2 including
(3)30 were	(17)7 using	(31)4 used	(45)2 indicate
(4)29 of	(18)6 from	(32)4 we	(46)2 investigated
(5)19 are	(19)6 published	(33)3 (GWAS)	(47)2 investigating
(6)19 that	(20)5 for	(34)3 demonstrated	(48)2 performed
(7)18 and	(21)5 revealed	(35)3 examining	(49)2 presented
(8)12 on	(22)5 utilizing	(36)3 involving	(50)2 provide
(9)9 in	(23)5 which	(37)2 among	(51)2 provided
(10)9 met	(24)4 assessing	(38)2 based	(52)2 related
(11)9 to	(25)4 evaluating	(39)2 established	(53)2 reporting
(12)8 reported	(26)4 however,	(40)2 examined
(13)8 with	(27)4 included	(41)2 focusing
(14)7 conducted	(28)4 show	(42)2 found

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--- WordNet output for studies --- Overview of noun study The noun study has 10 senses (first 8 from tagged texts) 1. (90) survey, study -- (a detailed critical inspection) 2. (17) study, work -- (applying the mind to learning and understanding a subject (especially by reading); "mastering a second language requires a lot of work"; "no schools offer graduate study in interior design") 3. (6) report, study, written report -- (a written document describing the findings of some individual or group; "this accords with the recent study by Hill and Dale") 4. (6) study -- (a state of deep mental absorption; "she is in a deep study") 5. (6) study -- (a room used for reading and writing and studying; "he knocked lightly on the closed door of the study") 6. (3) discipline, subject, subject area, subject field, field, field of study, study, bailiwick -- (a branch of knowledge; "in what discipline is his doctorate?"; "teachers should be well trained in their subject"; "anthropology is the study of human beings") 7. (2) sketch, study -- (preliminary drawing for later elaboration; "he made several studies before starting to paint") 8. (1) cogitation, study -- (attentive consideration and meditation; "after much cogitation he rejected the offer") 9. study -- (someone who memorizes quickly and easily (as the lines for a part in a play); "he is a quick study") 10. study -- (a composition intended to develop one aspect of the performer's technique; "a study in spiccato bowing") Overview of verb study The verb study has 6 senses (first 6 from tagged texts) 1. (73) analyze, analyse, study, examine, canvass, canvas -- (consider in detail and subject to an analysis in order to discover essential features or meaning; "analyze a sonnet by Shakespeare"; "analyze the evidence in a criminal trial"; "analyze your real motives") 2. (17) study -- (be a student; follow a course of study; be enrolled at an institute of learning) 3. (15) study, consider -- (give careful consideration to; "consider the possibility of moving") 4. (13) learn, study, read, take -- (be a student of a certain subject; "She is reading for the bar exam") 5. (5) study, hit the books -- (learn by reading books; "He is studying geology in his room"; "I have an exam next week; I must hit the books now") 6. (2) study, meditate, contemplate -- (think intently and at length, as for spiritual purposes; "He is meditating in his study") --- WordNet end ---