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138) In this study, we report a quick and simple method for comparing decellularization protocols combining the use of paraffin slices and two-dimensional cell cultures.
2015 Journal of tissue engineering and regenerative medicine
* Dewaxed ECM: A simple method for analyzing cell behaviour on decellularized extracellular matrices.
- Decellularization techniques have been used on a wide variety of tissues to create cell-seedable scaffolds for tissue engineering. Finding a suitable decellularization protocol for a certain type of tissue can be laborious, especially when organ perfusion devices are needed. In this study, we report a quick and simple method for comparing decellularization protocols combining the use of paraffin slices and two-dimensional cell cultures. We developed three decellularization protocols for adult murine kidney that yielded decellularized extracellular matrices (ECMs) with varying histological properties. The resulting paraffin-embedded ECM slices were deparaffinized and reseeded with murine embryonic stem cells (mESCs). We analyzed cell attachment four days post seeding via determination of cell numbers, and used quantitative Real-Time PCR 13 days post seeding to measure gene expression levels of two genes associated with renal development, Pax2 and Pou3f3. The three decellularization protocols produced kidney-matrices that showed clearly distinguishable results. We demonstrated that formerly paraffin-embedded decellularized ECMs can effectively influence differentiation of stem cells. This method can be used to identify optimal decellularization protocols for recellularization of three-dimensional tissue-scaffolds with embryonic stem cells and other tissue-specific cell types.
=>1.〜を勉強[研究]する, 学ぶ, 調べる, 2.調査, 勉強, 3.書斎, 勉強する
Overview of noun study
The noun study has 10 senses (first 8 from tagged texts)
1. (90) survey, study -- (a detailed critical inspection)
2. (17) study, work -- (applying the mind to learning and understanding a subject (especially by
reading); "mastering a second language requires a lot of work"; "no schools offer graduate study in
interior design")
3. (6) report, study, written report -- (a written document describing the findings of some
individual or group; "this accords with the recent study by Hill and Dale")
4. (6) study -- (a state of deep mental absorption; "she is in a deep study")
5. (6) study -- (a room used for reading and writing and studying; "he knocked lightly on the closed
door of the study")
6. (3) discipline, subject, subject area, subject field, field, field of study, study, bailiwick --
(a branch of knowledge; "in what discipline is his doctorate?"; "teachers should be well trained in
their subject"; "anthropology is the study of human beings")
7. (2) sketch, study -- (preliminary drawing for later elaboration; "he made several studies before
starting to paint")
8. (1) cogitation, study -- (attentive consideration and meditation; "after much cogitation he
rejected the offer")
9. study -- (someone who memorizes quickly and easily (as the lines for a part in a play); "he is a
quick study")
10. study -- (a composition intended to develop one aspect of the performer's technique; "a study in
spiccato bowing")
Overview of verb study
The verb study has 6 senses (first 6 from tagged texts)
1. (73) analyze, analyse, study, examine, canvass, canvas -- (consider in detail and subject to an
analysis in order to discover essential features or meaning; "analyze a sonnet by Shakespeare";
"analyze the evidence in a criminal trial"; "analyze your real motives")
2. (17) study -- (be a student; follow a course of study; be enrolled at an institute of learning)
3. (15) study, consider -- (give careful consideration to; "consider the possibility of moving")
4. (13) learn, study, read, take -- (be a student of a certain subject; "She is reading for the bar
exam")
5. (5) study, hit the books -- (learn by reading books; "He is studying geology in his room"; "I
have an exam next week; I must hit the books now")
6. (2) study, meditate, contemplate -- (think intently and at length, as for spiritual purposes; "He
is meditating in his study")
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