* Optimization of carboxylate-terminated poly(amidoamine) dendrimer-mediated cisplatin formulation.
- Abstract Cisplatin is mainly used in the treatment of ovarian, head and neck and testicular cancer. Poor solubility and non-specific interactions causes hurdles in the development of successful cisplatin formulation. There were few reports on poly(amidoamine) (PAMAM) dendrimer-cisplatin complexes for anticancer treatment. But the earlier research was mainly focused on therapeutic effect of PAMAM dendrimer-cisplatin complex, with less attention paid on the formulation development of these complexes. Objective of the present study is to optimize and validate the carboxylate-terminated, EDA core PAMAM dendrimer-based cisplatin formulation with respect to various variables such as dendrimer core, generation, drug entrapment, purification, yield, reproducibility, stability, storage and in-vitro release. Dendrimer-cisplatin complex was prepared by an efficient method which significantly increases the % platinum (Pt) content along with the product yield. Dendrimers showed reproducible (∼27%) platinum loading by weight. Variation in core and generations does not produce significant change in the % Pt content. Percentage Pt content of dendrimeric formulation increases with increase in drug/dendrimer mole ratio. Formulation with low drug/dendrimer mole ratio showed delayed release compared to the higher drug/dendrimer mole ratio; these dendrimer formulations are stable in room temperature. In vitro release profiles of the stored dendrimer-cisplatin samples showed comparatively slow release of cisplatin, which may be due to formation of strong bond between cisplatin and dendrimer. This study will contribute to create a fine print for the formulation development of PAMAM dendrimer-cisplatin complexes.
=>1.その場所で, そこに(で), そこへ, 2.〜がある, その場所で
Overview of noun there
The noun there has 1 sense (no senses from tagged texts)
1. there -- (a location other than here; that place; "you can take it from there")
Overview of adv there
The adv there has 3 senses (first 3 from tagged texts)
1. (181) there, at that place, in that location -- (in or at that place; "they have lived there for
years"; "it's not there"; "that man there")
2. (6) there, in that respect, on that point -- (in that matter; "I agree with you there")
3. (2) there, thither -- (to or toward that place; away from the speaker; "go there around noon!")
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