ELIZA

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296134 occurrences (No.80 in the rank) during 5 years in the PubMed. [no cache] 500 found

251) Unsupervised clustering of the transcriptional profiles of a total of 86,224 cells yielded 36 distinct clusters.

--- ABSTRACT ---

PMID:34035044 DOI:10.1101/gr.268037.120

2021 Genome research

* The Drosophila brain on cocaine at single-cell resolution.

- Whereas the neurological effects of cocaine have been well documented, effects of acute cocaine consumption on genome-wide gene expression across the brain remain largely unexplored. This question cannot be readily addressed in humans but can be approached using the Drosophila melanogaster model, where gene expression in the entire brain can be surveyed at once. Flies exposed to cocaine show impaired locomotor activity, including climbing behavior and startle response (a measure of sensorimotor integration), and increased incidence of seizures and compulsive grooming. To identify specific cell populations that respond to acute cocaine exposure, we analyzed single-cell transcriptional responses in duplicate samples of flies that consumed fixed amounts of sucrose or sucrose supplemented with cocaine, in both sexes. Unsupervised clustering of the transcriptional profiles of a total of 86,224 cells yielded 36 distinct clusters. Annotation of clusters based on gene markers revealed that all major cell types (neuronal and glial) as well as neurotransmitter types from most brain regions were represented. The brain transcriptional responses to cocaine showed profound sexual dimorphism and were considerably more pronounced in males than females. Differential expression analysis within individual clusters indicated cluster-specific responses to cocaine. Clusters corresponding to Kenyon cells of the mushroom bodies and glia showed especially large transcriptional responses following cocaine exposure. Cluster specific coexpression networks and global interaction networks revealed a diverse array of cellular processes affected by acute cocaine exposure. These results provide an atlas of sexually dimorphic cocaine-modulated gene expression in a model brain.

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(1)271 of	(8)4 volume	(15)2 WMS	(22)2 drug-related
(2)9 number	(9)3 costs	(16)2 active	(23)2 hemoglobin
(3)8 OH	(10)3 knee	(17)2 amount	(24)2 intracranial
(4)6 energy	(11)3 mean	(18)2 cholesterol	(25)2 plasma
(5)4 body	(12)3 radioactivity	(19)2 cholesterol,	(26)2 variation
(6)4 protein	(13)2 null	(20)2 clearance
(7)4 the	(14)2 72	(21)2 data

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--- WordNet output for total --- =>1.(車が)全壊する, 全損する, 2.全体の, 全ての, 総計の, 総体の, 全くの, 完全な, 総力の, 3.総額, 総計, 全体の, (車が)全壊する, 合計(する), なる Overview of noun total The noun total has 2 senses (first 2 from tagged texts) 1. (13) sum, total, totality, aggregate -- (the whole amount) 2. (6) sum, amount, total -- (a quantity obtained by the addition of a group of numbers) Overview of verb total The verb total has 3 senses (first 2 from tagged texts) 1. (16) total, number, add up, come, amount -- (add up in number or quantity; "The bills amounted to $2,000"; "The bill came to $2,000") 2. (2) total, tot, tot up, sum, sum up, summate, tote up, add, add together, tally, add up -- (determine the sum of; "Add all the people in this town to those of the neighboring town") 3. total -- (damage beyond the point of repair; "My son totaled our new car"; "the rock star totals his guitar at every concert") Overview of adj total The adj total has 2 senses (first 2 from tagged texts) 1. (31) entire, full, total -- (constituting the full quantity or extent; complete; "an entire town devastated by an earthquake"; "gave full attention"; "a total failure") 2. (2) full, total -- (complete in extent or degree and in every particular; "a full game"; "a total eclipse"; "a total disaster") --- WordNet end ---