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320) In vitro cell-based biochemical assays were performed to determine glycosaminoglycans (GAGs), DNA and total collagen contents from different PCL-alginate gel constructs.
2015 Journal of tissue engineering and regenerative medicine
* An additive manufacturing-based PCL-alginate-chondrocyte bioprinted scaffold for cartilage tissue engineering.
- Regenerative medicine is targeted to improve, restore or replace damaged tissues or organs using a combination of cells, materials and growth factors. Both tissue engineering and developmental biology currently deal with the process of tissue self-assembly and extracellular matrix (ECM) deposition. In this investigation, additive manufacturing (AM) with a multihead deposition system (MHDS) was used to fabricate three-dimensional (3D) cell-printed scaffolds using layer-by-layer (LBL) deposition of polycaprolactone (PCL) and chondrocyte cell-encapsulated alginate hydrogel. Appropriate cell dispensing conditions and optimum alginate concentrations for maintaining cell viability were determined. In vitro cell-based biochemical assays were performed to determine glycosaminoglycans (GAGs), DNA and total collagen contents from different PCL-alginate gel constructs. PCL-alginate gels containing transforming growth factor-β (TGFβ) showed higher ECM formation. The 3D cell-printed scaffolds of PCL-alginate gel were implanted in the dorsal subcutaneous spaces of female nude mice. Histochemical [Alcian blue and haematoxylin and eosin (H&E) staining] and immunohistochemical (type II collagen) analyses of the retrieved implants after 4 weeks revealed enhanced cartilage tissue and type II collagen fibril formation in the PCL-alginate gel (+TGFβ) hybrid scaffold. In conclusion, we present an innovative cell-printed scaffold for cartilage regeneration fabricated by an advanced bioprinting technology.
=>1.(車が)全壊する, 全損する, 2.全体の, 全ての, 総計の, 総体の, 全くの, 完全な, 総力の, 3.総額, 総計,
全体の, (車が)全壊する, 合計(する), なる
Overview of noun total
The noun total has 2 senses (first 2 from tagged texts)
1. (13) sum, total, totality, aggregate -- (the whole amount)
2. (6) sum, amount, total -- (a quantity obtained by the addition of a group of numbers)
Overview of verb total
The verb total has 3 senses (first 2 from tagged texts)
1. (16) total, number, add up, come, amount -- (add up in number or quantity; "The bills amounted to
$2,000"; "The bill came to $2,000")
2. (2) total, tot, tot up, sum, sum up, summate, tote up, add, add together, tally, add up --
(determine the sum of; "Add all the people in this town to those of the neighboring town")
3. total -- (damage beyond the point of repair; "My son totaled our new car"; "the rock star totals
his guitar at every concert")
Overview of adj total
The adj total has 2 senses (first 2 from tagged texts)
1. (31) entire, full, total -- (constituting the full quantity or extent; complete; "an entire town
devastated by an earthquake"; "gave full attention"; "a total failure")
2. (2) full, total -- (complete in extent or degree and in every particular; "a full game"; "a total
eclipse"; "a total disaster")
--- WordNet end ---